An annotated checklist of the species of non-marine molluscs from the Alburni Mountains (Salerno ... more An annotated checklist of the species of non-marine molluscs from the Alburni Mountains (Salerno Province, Campania, Southern Italy) is reported. The research was carried out from 2010 to 2013 inside a Site of Community Importance (SCI) and a Special Protection Area (SPA), of the Cilento, Vallo di Diano and Alburni National Park. The non-marine molluscs sampled on the field were compared with data available from the literature and malacological collections. Up to now, only 12 non-marine Mollusc species were known from the Alburni Mountains through bibliographical data. In all, the malacofauna of Alburni Mountains is composed by 83 non-marine Mollusc species (73 species of land snails, and 10 species of freshwater molluscs). The presence of nine species (six species of land snails and three species of freshwater snails) was confirmed by our field investigation, four species (3 species of land snails and 1 species of allochthonous freshwater snails) were recorded only by bibliographical data and were not yet found. Our analysis identifies 70 species of non-marine Molluscs (64 species of land snails, 6 species of freshwater molluscs) recorded on the basis of field data which were not previously recorded from the study area. At least 11 species are new records for the Campania Region. Extremely interesting is the record of Vertigo angustior Jeffreys, 1830 a species protected in European Union by the Annex II of the "Habitats Directive" and listed as "Vulnerable" at the European level. A Red List of Threatened Species is proposed and the species were classified with the code of I.U.C.N. (Version 2014.3). Five allochthonous species were surveyed for the first time in the study area: 3 land snails:
In Italy, the Eurasian otter, Lutra lutra (Linnaeus, 1758) (Carnivora Mustelidae), occurs with tw... more In Italy, the Eurasian otter, Lutra lutra (Linnaeus, 1758) (Carnivora Mustelidae), occurs with two separated populations in the southern regions and recently it has also re-colonized the north-eastern fluvial networks. Here, we present the first photographic evidence of the Eurasian otter presence along a stretch of the Calore river (Campania region, Southern Italy), where this species was generically reported in previous study. Our findings enlighten that the Calore river network might play an important role for the conservation and expansion of this species in Campania.
Iron-sulfur (Fe-S) clusters are inorganic cofactors that are fundamental to several biological pr... more Iron-sulfur (Fe-S) clusters are inorganic cofactors that are fundamental to several biological processes in all three kingdoms of life. In most organisms, Fe-S clusters are initially assembled on a scaffold protein, ISCU, and subsequently transferred to target proteins or to intermediate carriers by a dedicated chaperone/co-chaperone system. The delivery of assembled Fe-S clusters to recipient proteins is a crucial step in the biogenesis of Fe-S proteins, and, in mammals, it relies on the activity of a multiprotein transfer complex that contains the chaperone HSPA9, the co-chaperone HSC20 and the scaffold ISCU. How the transfer complex efficiently engages recipient Fe-S target proteins involves specific protein interactions that are not fully understood. This mini review focuses on recent insights into the molecular mechanism of amino acid motif recognition and discrimination by the co-chaperone HSC20, which guides Fe-S cluster delivery.
The major goal of evolutionary thermal biology is to understand how variation in temperature shap... more The major goal of evolutionary thermal biology is to understand how variation in temperature shapes phenotypic evolution. Comparing thermal reaction norms among populations from different thermal environments allows us to gain insights into the evolutionary mechanisms underlying thermal adaptation. Here, we have examined thermal adaptation in six wild populations of the fruit fly (Drosophila melanogaster) from markedly different natural environments by analyzing thermal reaction norms for fecundity, thorax length, wing area, and ovariole number under ecologically realistic fluctuating temperature regimes in the laboratory. Contrary to expectation, we found only minor differences in the thermal optima for fecundity among populations. Differentiation among populations was mainly due to differences in absolute (and partly also relative) thermal fecundity performance. Despite significant variation among populations in the absolute values of morphological traits, we observed only minor d...
The life history of the fruit fly (Drosophila melanogaster) is well understood, but fitness compo... more The life history of the fruit fly (Drosophila melanogaster) is well understood, but fitness components are rarely measured by following single individuals over their lifetime, thereby limiting insights into lifetime reproductive success, reproductive senescence and post‐reproductive lifespan. Moreover, most studies have examined long‐established laboratory strains rather than freshly caught individuals and may thus be confounded by adaptation to laboratory culture, inbreeding or mutation accumulation. Here, we have followed the life histories of individual females from three recently caught, non‐laboratory‐adapted wild populations of D. melanogaster. Populations varied in a number of life‐history traits, including ovariole number, fecundity, hatchability and lifespan. To describe individual patterns of age‐specific fecundity, we developed a new model that allowed us to distinguish four phases during a female's life: a phase of reproductive maturation, followed by a period of lin...
bioRxiv (Cold Spring Harbor Laboratory), Nov 10, 2020
Many complex genomic rearrangements arise through template switch errors, which occur in DNA repl... more Many complex genomic rearrangements arise through template switch errors, which occur in DNA replication when there is a transient polymerase switch to an alternate template nearby in three-dimensional space. While typically investigated at kilobase-to-megabase scales, the genomic and evolutionary consequences of this mutational process are not well characterised at smaller scales, where they are often interpreted as clusters of independent substitutions, insertions and deletions. Here we present an improved statistical approach using pair hidden Markov models, and use it to detect and describe short-range template switches underlying clusters of mutations in the multiway alignment of hominid genomes. Using robust statistics derived from evolutionary genomic simulations, we show that template switch events have been widespread in the evolution of the great apes' genomes and provide a parsimonious explanation for the presence of many complex mutation clusters in their phylogenetic context. Larger-scale mechanisms of genome rearrangement are typically associated with structural features around breakpoints, and accordingly we show that atypical patterns of secondary structure formation and DNA bending are present at the initial template switch loci. Our methods improve on previous non-probabilistic approaches for computational detection of template switch mutations, allowing the statistical significance of events to be assessed. By specifying realistic evolutionary parameters based on the genomes and taxa involved, our methods can be readily adapted to other intra-or inter-species comparisons.
Eobania vermiculata as a potential indicator of nitrate contamination in soil
Ecotoxicology and environmental safety, 2020
The effects of nitrates were analysed on the land snail Eobania vermiculata, a good bioindicator ... more The effects of nitrates were analysed on the land snail Eobania vermiculata, a good bioindicator to assess the effects of certain pollutants in soil. It is known that the molluscs are very sensitive to contamination substances and can be used as sentinel organism for environmental pollution assessment. The nitrates are present in fertilizers and in food additives and their excess can not only be harmful to the environment but also dangerous for the humans. Indeed, in the mammals the nitrates are converted into nitrites and can cause a series of complications as the formation of methaemoglobin and cancers. In this study, adult organisms of E. vermiculata were exposed to soil containing 2000 mg/L of nitrates for 30 days to evaluate the stool microbiome and the histological changes at the level of the foot. Eggs of these snails were similarly treated to observe their hatching, survival and development. Histological changes were observed at level of the foot of adult snails exposed to n...
Early evidence for a virus-like agent infecting the pest snail Theba pisana (Gastropoda: Pulmonata) in Southern Italy
Journal of invertebrate pathology, 2017
The Mediterranean land snail Theba pisana (Mollusca: Helicidae) is an introduced agricultural pes... more The Mediterranean land snail Theba pisana (Mollusca: Helicidae) is an introduced agricultural pest in many countries around the world, including Australia, Israel, USA and South Africa. In addition, this snail is an intermediate host of parasites of importance in both human and veterinary medicine. In this study, a natural population of T. pisana snails on the Domitian coast of Italy was surveyed following a mass mortality event. By light microscopy, 30% of the collected individuals showed in the calcium cells of the digestive gland the presence of hypertrophied nuclei containing eosinophilic to weakly basophilic inclusion bodies. Ultrastructural examination by transmission electron microscopy (TEM) showed nuclear inclusions constituted by a reticulated stroma into which unenveloped, roundish virus-like particles (38±4nm in diameter) were present. To the best of our knowledge this could be the first evidence for a virus-like agent infecting the gastropod T. pisana, which may open ne...
Molecular cytogenetic study of three common Mediterranean limpets, Patella caerulea, P. rustica and P. ulyssiponensis (Archaeogastropoda, Mollusca)
Genetica, 2009
The present paper shows the results of chromosome banding and rDNA-FISH study performed on severa... more The present paper shows the results of chromosome banding and rDNA-FISH study performed on several specimens of different populations of Patella caerulea, Patellarustica and Patellaulyssiponensis. The taxonomic attribution of specimens was ascertained by the molecular phylogenetic analysis of the mitochondrial 16S rRNA gene. P. caerulea and P. rustica had 2n = 18 chromosomes with first seven of biarmed pairs and the remaining two uniarmed pairs. P. ulyssiponensis had 2n = 16 with all biarmed chromosomes. Ag-NOR loci were on the short arms of the first metacentric pair in the three studied limpets, whereas they showed a different pattern of heterochromatin distribution and composition. A chromosome mosaicism was observed in several P. caerulea specimens, which exhibited an unpaired metacentric element and loss of a telocentric pair. The obtained results suggest that in the genus Patella specific diversification was accompanied by variations in heterochromatin distribution and composi...
Bufo viridis (Bonnaterre, 1789) (Rospo smeraldino), B. balearicus (Boettger, 1881) (Rospo smeraldino italiano), B. boulengeri Lataste, 1879 (Rospo smeraldino nordafricano), B. siculus (Stöck et al., 2008) (Rospo smeraldino siciliano)
Caretta caretta (Linnaeus, 1758)
I chirotteri del Parco Nazionale dev Visuvio e delle aree limitrofe: osservazioni faunistriche e considerazioni critiche sui dati storici
Atti della Società italiana di scienze naturali e del museo civico di storia naturale di Milano, 2001
I pinnipedi e i sirenii del Museo Zoologico dell'Universita di Napoli Federico II (Mammalia: Carnivora, Sirenia). Catalogo della collezione con note storiche e osteometriche
Atti della Società Italiana di Scienze Naturali e del Museo Civico di Storia Naturale in Milano., 2000
Nel corso di una ricerca sulla vita privata e professionale di Stefano delle Chiaje, lo studio di... more Nel corso di una ricerca sulla vita privata e professionale di Stefano delle Chiaje, lo studio di alcuni documenti ha fatto supporre che la Balenottera Minore esposta nel Museo Zoologico di Napoli fosse quella donata dallo scienziato al Gabinetto di Anatomia Patologica dell’Università (GAGP) nel 1848 e segnalata all’epoca dalla apposizione di una targa – Dono di Stefano delle Chiaje – grazie al permesso ottenuto, in riconoscenza del donativo, direttamente dal Re. In questo lavoro viene riportato il risultato di indagini effettuate sulla collezione cetologica del GAGP raccolta da Stefano delle Chiaje durante la sua direzione. Tale collezione risultava essere composta da almeno 13 reperti appartenenti ad almeno quattro specie di Cetacei: otto reperti di Balaenoptera acutorostrata, 6 dei quali (cranio e parte dello scheletro assile, sterno, osso ioide, rudimenti pelvici, bulle timpaniche e fanoni montati su base) riconducibili ad un individuo e due fanoni appartenenti ad un altro esem...
We investigated the relationship between age and body length, and age at sexual maturity of Physe... more We investigated the relationship between age and body length, and age at sexual maturity of Physeter macrocephalus individuals stranded along the Italian coast. Our molecular analysis shows that all our samples belong to the C.001.002 haplotype, shared between Atlantic and Mediterranean populations. We show that males attain sexual maturity at 10 years, similar to those from other marine areas. However, considering the same body length class, Mediterranean males are older than Atlantic ones. Our finding of a Mediterranean pregnant female of only 6.5 m in length and an assessed age of 24–26 years is particularly noteworthy, considering that females reach sexual maturity at about 9 years and 9 m of total length in other regions. Comparing our results with the literature data, we highlight the positive correlation between lifespan, adult body length and weight of males from the Mediterranean and Atlantic Ocean. Regardless of whether the relatively small size of Mediterranean specimens ...
Nothing is as it seems: genetic analyses on stranded fin whales unveil the presence of a fin-blue whale hybrid in the Mediterranean Sea (Balaenopteridae)
The Mediterranean Sea hosts a population of fin whale (Balaenoptera physalus), the only species o... more The Mediterranean Sea hosts a population of fin whale (Balaenoptera physalus), the only species of Mysticete regularly occurring in the basin. Observed and inferred mortality suggests that the population is likely declining. Accordingly, understanding the causes of mortality and assessing the health status is pivotal to the survival of this endangered population. While such studies are inherently difficult for a highly roaming species with a pelagic distribution, mortality events provide the opportunity to investigate biological and epidemiological traits linked to these events, and evaluate the footprint of human activity, especially when long-term data series exist. We present a comprehensive spatial–temporal overview of fin whale mortality events along the Italian coast encompassing four centuries (1624–2021). Time series analysis was used to highlight structural changes in the evolution of mortality through time, while spatial–temporal patterns in the distribution of mortality e...
Information on demographic and life-history traits of endangered vertebrate species, such as sea ... more Information on demographic and life-history traits of endangered vertebrate species, such as sea turtles, is crucial for planning management and conservation actions. We applied skeletochronology of phalanges to estimate the age of loggerhead turtles, Caretta caretta, found dead stranded along the beaches of Campania (western Mediterranean) from 2013 to 2017. To obtain maturity data, we examined gonads from a subsample of 7 males and 11 females. Overall, curved carapace length (CCL) ranged from 5.6 to 90.8 cm, but for most turtles (89%) it was 50-79.9 cm. Predominance of stranded females (62%) was recorded. Ten out of the eighteen histologically examined gonads allowed estimating maturity. Based on the lines of arrested growth counting, the estimated age of the examined specimens ranged from 0 (hatchling) to 26 years. The modal age was 14 years for males and 17 years for females. The smallest male with spermatogenetic activity had a CCL of 65 cm and was estimated to be 16 years old. The smallest female with follicular development stage, characterising the transition towards adulthood, had a CCL of 69.5 cm and was estimated to be 20 years old. Anthropogenic factors were responsible for 36% of the mortality of individuals, followed by parasitic/infective pathologies (20%).
We describe the karyotype, location of nucleolus-organizing regions (NORs) and heterochromatin co... more We describe the karyotype, location of nucleolus-organizing regions (NORs) and heterochromatin composition and distribution in Lepidochitona caprearum (Scacchi, 1836). The examined specimens had 2n=24 chromosomes; the elements of pairs 1-4 were metacentric, subtelocentric those of the fifth pair, telocentric the elements of other pairs. NOR-FISH, Ag-NOR-and CMA 3 banding showed NORs localized on pericentromeric regions of a medium small sized, telocentric chromosome pair. After C-banding or digestions with restriction enzyme NOR associate heterochromatin only was cytologically evident, resulting CMA 3 positive. The comparison with chromosome data of other chitons, other than to evidence a karyotypic similarity of L. caprearum to species of suborder Acanthochitonina, allows us to infer that chromosome evolution in the suborder mainly occurred via reduction of the number of the chromosomes by centric fusions, which took place repeatedly and independently in the different lineages of Acanthochitonina.
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Papers by Nicola Maio